Day 7
July 14
Location: The Big Obsidian Flow
This morning we did our analysis under the microscope
and collected more data from the Upper Deschutes River. We also worked together
on our field notes and made sure we all had the data we have collected and
organized it systematically. We went to the Big Obsidian Flow and did the 3/4 mile hike.
Photographed: View of the Big Obsidian Flow
Microscope Analysis: Strawberry Lake Gram
Observations I noted for my slide were:
-
Purple stain
o
Eggs, circular shapes, with clear
center
o
Vein like stains, some short some
longer than others
o
Empty pockets where no staining or
bacteria is present (minimal and small areas)
Field Journal:
Site 1
-
Strawberry Lake location
Date: July 9th, 2023
Time: 2:00 pm
Coordinates: 44.3068 Degrees North, 118.6852 Degrees
West
Elevation: 1800 m
Samples: 2 samples taken by Zach and Corbin in 50mL sterile
conical tubes
Site Description:
- The area is a lake from a glacial
valley surrounded by high walls. We did not take the depth but was
approximately over 6ft of water the further in you went. Zach and Corbin stated
that the water was cold but not as cold as the upper Deschutes River (50.4 F),
their observation also was that the turbidity of the water was lower in the
upper lake at this site. We were at a high elevation and noticed that the area
has a lot of disturbances from people, their pets and poor camping practices.
Site 2
-
Keyes Creek at OSU Geological Field
Station
Date: July 11th, 2023
Time: 9:07 am
Coordinates: 44 Degrees 33’ 59’’ N, 120 Degrees 6’28’’
W
Elevation: 3240m (2777m, Mitchell OR)
Samples: 3 samples taken by Jordon (2) and Ayana (1)
in 50mL sterile conical tubes
Site Description:
-
This was a very small creek approximately
4ft wide and had a thick canopy cover. The water was shallow and is a heavily
impacted side. Located along side a highway, having a pig farm just up the road
from it and the OSU students are here 4 weeks out of the year.
Site 3
-
Upper Deschutes River
Date: July 14th, 2023
Time: 9:22 am
Coordinates: 10T0598384, UTM4858322
Elevation: 1,064m
Samples: 3 samples taken by Jordon (2) and Ayana (1)
in 50mL sterile conical tubes
Site Description:
-
The bank was a wet marshland with
overgrown foliage. The rocks near the bank were very course and sand like while
the middle of the river had cobble-sized rocks with patches of algae present.
It is close to a road and has stairs that lead down to the bank where we
sampled from. It is in the Deschutes National Forests and the South-West side,
from which we collected water from, receives the morning sunlight. It was also
noted that snakes are present in the area.
We will be using the same sample processing method for
each of the samples of water we collect at various sources.
Sample Processing: Water Quality Assessment
-
Bacterial Indicator Species
o
Coliform and/or other types of bacteria
that can grow on selective media used
-
Selective Media Used
o
NUT-TTC
o
MAC
-
Bacterial Culturing (Process)
o
Single use inoculation loop to
isolate a single species of bacteria
o
Streaked single colony onto
nutrient agar plate
o
Placed agar plate into incubator
for an additional 36-48 hours
-
Slide Prep
o
Labeled slide with
§
Site
§
Initials
§
Date
§
Gram Stain
o
Bacterial Smear
§
Placed drop of water on slide when
utilized inoculation loop into the placed water adding a scraper from the agar
plate, let air dry
o
Heat fixation
§
Took slide and passed through Bunsen
burner at least 3x (Blue part of flame)
o
Bacterial Staining/Gram Stain
§
Placed the slide (with bacteria) on
slide rack over a disposable food container
§
Cover smear with crystal violet
for 20s
§
Rinse slide with distilled water
to remove excess crystal violet
§
Cover smear with gram iodine for 1
min
§
Rinse slide with distilled water
to remove excess gram iodine
§
Decolorized with ethanol until
color stopped running
§
Immediately rinse slide to remove
decoloring agent
§
Cover smear with Safranin for 1
min
§
Rinse slide with distilled water
to remove excess Safranin
§
Let air dry
§
Analyzed samples under microscope
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